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wild type brd2  (Addgene inc)


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    Addgene inc wild type brd2
    a , Effect of E 2 on gene expression with or without JQ1 treatment in MCF7 cells. Data represent log2 fold change in comparison of E 2 with vehicle treatment with or without JQ1 pre-treatment. Color shows statistical significance in the condition without JQ1 (purple), with JQ1 (green) or both settings (red). b,c, Immunoblot of indicated proteins with doses of dBET6, JQ1 or MZ1 treatments. The asterisk in b indicates an unknown band detected by the Abcam antibody that recognizes the N-terminus of BRD4. Cells in b and c were cultured in full medium (5% FBS, phenol-red). The asterisks in c indicate unknown bands. d , Effect of JQ1 (1 μM), MZ1 (500 nM) and NVP2 (250 nM) on ER-mediated gene transcription measured by qPCR. MCF7 cells were treated with inhibitors or degraders, as indicated, for 2 hours prior to a 2-hour induction by E 2 (10 nM). NVP2 is a CDK9 inhibitor that serves as a positive control. e-g , Immunoblot of indicated proteins in whole cell lysates of MCF7 cells expressing shRNAs for GFP (ctr), BRD4-L (424, 972), BRD4-S (7, 8, 9), BRD4 (4, 5, 6), <t>BRD2</t> (B, D), or BRD3 (E, F). The asterisk indicates an unknown band. h , Immunoblot of BRD4 expression in MCF7 FRT/Tet-on whole cell lysates. BRD4L-WT or BRD4 bromodomain mutant (BRD4L mut ) Tet-On cells were treated with doxycycline 100 ng/ml overnight prior to dBET6 treatment (500 nM) for 2 hours. i , Effect of different BET bromodomain mutants on ER-mediated gene transcription measured by qPCR. Tet-On 3G:BET-BD cells were treated with doxycycline 10 ng/ml overnight prior to dBET6 treatment for 2 hours followed by 1-hour induction by E 2 . Data are shown as mean ± s.d. from independent triplicates.
    Wild Type Brd2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type brd2/product/Addgene inc
    Average 93 stars, based on 16 article reviews
    wild type brd2 - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity"

    Article Title: Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity

    Journal: bioRxiv

    doi: 10.1101/2024.07.25.605008

    a , Effect of E 2 on gene expression with or without JQ1 treatment in MCF7 cells. Data represent log2 fold change in comparison of E 2 with vehicle treatment with or without JQ1 pre-treatment. Color shows statistical significance in the condition without JQ1 (purple), with JQ1 (green) or both settings (red). b,c, Immunoblot of indicated proteins with doses of dBET6, JQ1 or MZ1 treatments. The asterisk in b indicates an unknown band detected by the Abcam antibody that recognizes the N-terminus of BRD4. Cells in b and c were cultured in full medium (5% FBS, phenol-red). The asterisks in c indicate unknown bands. d , Effect of JQ1 (1 μM), MZ1 (500 nM) and NVP2 (250 nM) on ER-mediated gene transcription measured by qPCR. MCF7 cells were treated with inhibitors or degraders, as indicated, for 2 hours prior to a 2-hour induction by E 2 (10 nM). NVP2 is a CDK9 inhibitor that serves as a positive control. e-g , Immunoblot of indicated proteins in whole cell lysates of MCF7 cells expressing shRNAs for GFP (ctr), BRD4-L (424, 972), BRD4-S (7, 8, 9), BRD4 (4, 5, 6), BRD2 (B, D), or BRD3 (E, F). The asterisk indicates an unknown band. h , Immunoblot of BRD4 expression in MCF7 FRT/Tet-on whole cell lysates. BRD4L-WT or BRD4 bromodomain mutant (BRD4L mut ) Tet-On cells were treated with doxycycline 100 ng/ml overnight prior to dBET6 treatment (500 nM) for 2 hours. i , Effect of different BET bromodomain mutants on ER-mediated gene transcription measured by qPCR. Tet-On 3G:BET-BD cells were treated with doxycycline 10 ng/ml overnight prior to dBET6 treatment for 2 hours followed by 1-hour induction by E 2 . Data are shown as mean ± s.d. from independent triplicates.
    Figure Legend Snippet: a , Effect of E 2 on gene expression with or without JQ1 treatment in MCF7 cells. Data represent log2 fold change in comparison of E 2 with vehicle treatment with or without JQ1 pre-treatment. Color shows statistical significance in the condition without JQ1 (purple), with JQ1 (green) or both settings (red). b,c, Immunoblot of indicated proteins with doses of dBET6, JQ1 or MZ1 treatments. The asterisk in b indicates an unknown band detected by the Abcam antibody that recognizes the N-terminus of BRD4. Cells in b and c were cultured in full medium (5% FBS, phenol-red). The asterisks in c indicate unknown bands. d , Effect of JQ1 (1 μM), MZ1 (500 nM) and NVP2 (250 nM) on ER-mediated gene transcription measured by qPCR. MCF7 cells were treated with inhibitors or degraders, as indicated, for 2 hours prior to a 2-hour induction by E 2 (10 nM). NVP2 is a CDK9 inhibitor that serves as a positive control. e-g , Immunoblot of indicated proteins in whole cell lysates of MCF7 cells expressing shRNAs for GFP (ctr), BRD4-L (424, 972), BRD4-S (7, 8, 9), BRD4 (4, 5, 6), BRD2 (B, D), or BRD3 (E, F). The asterisk indicates an unknown band. h , Immunoblot of BRD4 expression in MCF7 FRT/Tet-on whole cell lysates. BRD4L-WT or BRD4 bromodomain mutant (BRD4L mut ) Tet-On cells were treated with doxycycline 100 ng/ml overnight prior to dBET6 treatment (500 nM) for 2 hours. i , Effect of different BET bromodomain mutants on ER-mediated gene transcription measured by qPCR. Tet-On 3G:BET-BD cells were treated with doxycycline 10 ng/ml overnight prior to dBET6 treatment for 2 hours followed by 1-hour induction by E 2 . Data are shown as mean ± s.d. from independent triplicates.

    Techniques Used: Gene Expression, Comparison, Western Blot, Cell Culture, Positive Control, Expressing, Mutagenesis

    a , Immunoblot of indicated proteins from co-IP of BET proteins in MCF7 nuclear extracts. 5 µM of (-)-JQ1 or (+)-JQ1 was added during immunoprecipitation. b , Immunoblot of indicated proteins in FLAG-PAF1-dTAG cells treated with or without 500 nM dTAG V -1 for 2 hours. c , qPCR analyses of gene expression changes in FLAG-PAF1-dTAG cells treated with or without JQ1, dTAG or both prior to E 2 . d , ChIP-qPCR analyses of Pol II (NTD) binding at gene bodies in FLAG-PAF1-dTAG cells under indicated conditions. E 2 was used in all treatments except for the vehicle group (V). Data shown as mean ± s.d. from independent triplicates. p values from two-tailed t-tests (compared to E 2 treatment alone) are depicted with asterisks. e , Proposed model of BET proteins in ER-mediated transcription. Note that BRD2 and BRD3 are not shown for conciseness. They can also associate with elongation factors to facilitate transcription. GTF, general transcription factors. See also Extended Data Fig.7.
    Figure Legend Snippet: a , Immunoblot of indicated proteins from co-IP of BET proteins in MCF7 nuclear extracts. 5 µM of (-)-JQ1 or (+)-JQ1 was added during immunoprecipitation. b , Immunoblot of indicated proteins in FLAG-PAF1-dTAG cells treated with or without 500 nM dTAG V -1 for 2 hours. c , qPCR analyses of gene expression changes in FLAG-PAF1-dTAG cells treated with or without JQ1, dTAG or both prior to E 2 . d , ChIP-qPCR analyses of Pol II (NTD) binding at gene bodies in FLAG-PAF1-dTAG cells under indicated conditions. E 2 was used in all treatments except for the vehicle group (V). Data shown as mean ± s.d. from independent triplicates. p values from two-tailed t-tests (compared to E 2 treatment alone) are depicted with asterisks. e , Proposed model of BET proteins in ER-mediated transcription. Note that BRD2 and BRD3 are not shown for conciseness. They can also associate with elongation factors to facilitate transcription. GTF, general transcription factors. See also Extended Data Fig.7.

    Techniques Used: Western Blot, Co-Immunoprecipitation Assay, Immunoprecipitation, Gene Expression, ChIP-qPCR, Binding Assay, Two Tailed Test



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    wild type brd2  (Addgene inc)
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    Addgene inc wild type brd2
    a , Effect of E 2 on gene expression with or without JQ1 treatment in MCF7 cells. Data represent log2 fold change in comparison of E 2 with vehicle treatment with or without JQ1 pre-treatment. Color shows statistical significance in the condition without JQ1 (purple), with JQ1 (green) or both settings (red). b,c, Immunoblot of indicated proteins with doses of dBET6, JQ1 or MZ1 treatments. The asterisk in b indicates an unknown band detected by the Abcam antibody that recognizes the N-terminus of BRD4. Cells in b and c were cultured in full medium (5% FBS, phenol-red). The asterisks in c indicate unknown bands. d , Effect of JQ1 (1 μM), MZ1 (500 nM) and NVP2 (250 nM) on ER-mediated gene transcription measured by qPCR. MCF7 cells were treated with inhibitors or degraders, as indicated, for 2 hours prior to a 2-hour induction by E 2 (10 nM). NVP2 is a CDK9 inhibitor that serves as a positive control. e-g , Immunoblot of indicated proteins in whole cell lysates of MCF7 cells expressing shRNAs for GFP (ctr), BRD4-L (424, 972), BRD4-S (7, 8, 9), BRD4 (4, 5, 6), <t>BRD2</t> (B, D), or BRD3 (E, F). The asterisk indicates an unknown band. h , Immunoblot of BRD4 expression in MCF7 FRT/Tet-on whole cell lysates. BRD4L-WT or BRD4 bromodomain mutant (BRD4L mut ) Tet-On cells were treated with doxycycline 100 ng/ml overnight prior to dBET6 treatment (500 nM) for 2 hours. i , Effect of different BET bromodomain mutants on ER-mediated gene transcription measured by qPCR. Tet-On 3G:BET-BD cells were treated with doxycycline 10 ng/ml overnight prior to dBET6 treatment for 2 hours followed by 1-hour induction by E 2 . Data are shown as mean ± s.d. from independent triplicates.
    Wild Type Brd2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type brd2/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    wild type brd2 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

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    a , Effect of E 2 on gene expression with or without JQ1 treatment in MCF7 cells. Data represent log2 fold change in comparison of E 2 with vehicle treatment with or without JQ1 pre-treatment. Color shows statistical significance in the condition without JQ1 (purple), with JQ1 (green) or both settings (red). b,c, Immunoblot of indicated proteins with doses of dBET6, JQ1 or MZ1 treatments. The asterisk in b indicates an unknown band detected by the Abcam antibody that recognizes the N-terminus of BRD4. Cells in b and c were cultured in full medium (5% FBS, phenol-red). The asterisks in c indicate unknown bands. d , Effect of JQ1 (1 μM), MZ1 (500 nM) and NVP2 (250 nM) on ER-mediated gene transcription measured by qPCR. MCF7 cells were treated with inhibitors or degraders, as indicated, for 2 hours prior to a 2-hour induction by E 2 (10 nM). NVP2 is a CDK9 inhibitor that serves as a positive control. e-g , Immunoblot of indicated proteins in whole cell lysates of MCF7 cells expressing shRNAs for GFP (ctr), BRD4-L (424, 972), BRD4-S (7, 8, 9), BRD4 (4, 5, 6), BRD2 (B, D), or BRD3 (E, F). The asterisk indicates an unknown band. h , Immunoblot of BRD4 expression in MCF7 FRT/Tet-on whole cell lysates. BRD4L-WT or BRD4 bromodomain mutant (BRD4L mut ) Tet-On cells were treated with doxycycline 100 ng/ml overnight prior to dBET6 treatment (500 nM) for 2 hours. i , Effect of different BET bromodomain mutants on ER-mediated gene transcription measured by qPCR. Tet-On 3G:BET-BD cells were treated with doxycycline 10 ng/ml overnight prior to dBET6 treatment for 2 hours followed by 1-hour induction by E 2 . Data are shown as mean ± s.d. from independent triplicates.

    Journal: bioRxiv

    Article Title: Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity

    doi: 10.1101/2024.07.25.605008

    Figure Lengend Snippet: a , Effect of E 2 on gene expression with or without JQ1 treatment in MCF7 cells. Data represent log2 fold change in comparison of E 2 with vehicle treatment with or without JQ1 pre-treatment. Color shows statistical significance in the condition without JQ1 (purple), with JQ1 (green) or both settings (red). b,c, Immunoblot of indicated proteins with doses of dBET6, JQ1 or MZ1 treatments. The asterisk in b indicates an unknown band detected by the Abcam antibody that recognizes the N-terminus of BRD4. Cells in b and c were cultured in full medium (5% FBS, phenol-red). The asterisks in c indicate unknown bands. d , Effect of JQ1 (1 μM), MZ1 (500 nM) and NVP2 (250 nM) on ER-mediated gene transcription measured by qPCR. MCF7 cells were treated with inhibitors or degraders, as indicated, for 2 hours prior to a 2-hour induction by E 2 (10 nM). NVP2 is a CDK9 inhibitor that serves as a positive control. e-g , Immunoblot of indicated proteins in whole cell lysates of MCF7 cells expressing shRNAs for GFP (ctr), BRD4-L (424, 972), BRD4-S (7, 8, 9), BRD4 (4, 5, 6), BRD2 (B, D), or BRD3 (E, F). The asterisk indicates an unknown band. h , Immunoblot of BRD4 expression in MCF7 FRT/Tet-on whole cell lysates. BRD4L-WT or BRD4 bromodomain mutant (BRD4L mut ) Tet-On cells were treated with doxycycline 100 ng/ml overnight prior to dBET6 treatment (500 nM) for 2 hours. i , Effect of different BET bromodomain mutants on ER-mediated gene transcription measured by qPCR. Tet-On 3G:BET-BD cells were treated with doxycycline 10 ng/ml overnight prior to dBET6 treatment for 2 hours followed by 1-hour induction by E 2 . Data are shown as mean ± s.d. from independent triplicates.

    Article Snippet: Wild-type BRD2 and BRD3 from GFP-BRD2 (Addgene 65376) and GFP-BRD3 (Addgene 65377) from Kyle Miller were cloned into the pcDNA5/FRT/TO vector.

    Techniques: Gene Expression, Comparison, Western Blot, Cell Culture, Positive Control, Expressing, Mutagenesis

    a , Immunoblot of indicated proteins from co-IP of BET proteins in MCF7 nuclear extracts. 5 µM of (-)-JQ1 or (+)-JQ1 was added during immunoprecipitation. b , Immunoblot of indicated proteins in FLAG-PAF1-dTAG cells treated with or without 500 nM dTAG V -1 for 2 hours. c , qPCR analyses of gene expression changes in FLAG-PAF1-dTAG cells treated with or without JQ1, dTAG or both prior to E 2 . d , ChIP-qPCR analyses of Pol II (NTD) binding at gene bodies in FLAG-PAF1-dTAG cells under indicated conditions. E 2 was used in all treatments except for the vehicle group (V). Data shown as mean ± s.d. from independent triplicates. p values from two-tailed t-tests (compared to E 2 treatment alone) are depicted with asterisks. e , Proposed model of BET proteins in ER-mediated transcription. Note that BRD2 and BRD3 are not shown for conciseness. They can also associate with elongation factors to facilitate transcription. GTF, general transcription factors. See also Extended Data Fig.7.

    Journal: bioRxiv

    Article Title: Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity

    doi: 10.1101/2024.07.25.605008

    Figure Lengend Snippet: a , Immunoblot of indicated proteins from co-IP of BET proteins in MCF7 nuclear extracts. 5 µM of (-)-JQ1 or (+)-JQ1 was added during immunoprecipitation. b , Immunoblot of indicated proteins in FLAG-PAF1-dTAG cells treated with or without 500 nM dTAG V -1 for 2 hours. c , qPCR analyses of gene expression changes in FLAG-PAF1-dTAG cells treated with or without JQ1, dTAG or both prior to E 2 . d , ChIP-qPCR analyses of Pol II (NTD) binding at gene bodies in FLAG-PAF1-dTAG cells under indicated conditions. E 2 was used in all treatments except for the vehicle group (V). Data shown as mean ± s.d. from independent triplicates. p values from two-tailed t-tests (compared to E 2 treatment alone) are depicted with asterisks. e , Proposed model of BET proteins in ER-mediated transcription. Note that BRD2 and BRD3 are not shown for conciseness. They can also associate with elongation factors to facilitate transcription. GTF, general transcription factors. See also Extended Data Fig.7.

    Article Snippet: Wild-type BRD2 and BRD3 from GFP-BRD2 (Addgene 65376) and GFP-BRD3 (Addgene 65377) from Kyle Miller were cloned into the pcDNA5/FRT/TO vector.

    Techniques: Western Blot, Co-Immunoprecipitation Assay, Immunoprecipitation, Gene Expression, ChIP-qPCR, Binding Assay, Two Tailed Test